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LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet.

ABSTRACT: This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.

SUBMITTER: Usukura E

PROVIDER: S-EPMC5731743 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet.

Usukura Eiji E Yanase Yuhki Y Ishijima Ayumi A Kuboki Thasaneeya T Kidoaki Satoru S Okamoto Koichi K Tamada Kaoru K

PloS one 20171215 12

This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides com ...[more]

PMID: 29244869

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