Dataset Information

Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil.

ABSTRACT:

Background

Some sexually transmitted infectious agents, such as Chlamydia trachomatis and Herpes simplex, cause local inflammation, and could contribute to Human Papillomavirus (HPV) and cervical lesion progression. Thus, the aim of this study was to determine any association between the presence of microorganisms of gynecological importance, sexual behavior, clinical and demographical variables to the development and progress of cervical lesions.

Methods

One hundred and thirty-two women between 14 and 78 years and living at Vitória da Conquista, Bahia, Brazil, were included (62 individuals with cervical lesions and 70 without lesions). They answered a questionnaire to provide data for a socioeconomic and sexual activity profile. Samples of cervical swabs were collected and analyzed by PCR to detect genital microorganisms and HPV. Quantitative PCR was used to detect and quantify Ureaplasma urealyticum and Ureaplasma parvum. Univariate and multiple logistic regression were performed to measure the association with the cervical lesions, and an odds ratio (OR) with 95% confidence intervals (95%CI) were calculated. The Mann-Whitney U test was also used to compare the microorganism load in the case and control groups. The significance level was 5% in all hypotheses tested.

Results

Cervical lesions were associated with: women in a stable sexual relationship (OR?=?14.21, 95%CI?=?3.67-55.018), positive PCR for HPV (OR?=?16.81, 95%CI?=?4.19-67.42), Trichomonas vaginalis (OR?=?8.566, 95%CI?=?2.04-35.94) and Gardnerella vaginalis (OR?=?6.13, 95%CI?=?1.53-24.61), adjusted by age and qPCR for U. parvum. U. parvum load showed a statistical difference between the case and control groups (p-value?=?0.002).

Conclusion

Variables such as stable relationship, HPV, T. vaginalis, G. vaginalis were associated with cervical lesions in epidemiological studies. U. parvum load was higher in woman with cervical lesions compared with women without lesions. Additional studies are needed to better understand the role of these factors in cervical lesion development.

SUBMITTER: Amorim AT

PROVIDER: S-EPMC5732421 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Publications

Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil.

Amorim Aline Teixeira AT Marques Lucas Miranda LM Campos Guilherme Barreto GB Lobão Tássia Neves TN de Souza Lino Vanesca V Cintra Ricardo Cesar RC Andreoli Maria Antonieta MA Villa Luisa Lina LL Boccardo Enrique E Junior Antonio Carlos Ricardo Braga ACRB López Rossana Verónica Mendoza RVM Dos Santos Djanilson Barbosa DB de Souza Gerson Maciel GM Romano Carla Cristina CC Timenetsky Jorge J

BMC infectious diseases 20171215 1

<h4>Background</h4>Some sexually transmitted infectious agents, such as Chlamydia trachomatis and Herpes simplex, cause local inflammation, and could contribute to Human Papillomavirus (HPV) and cervical lesion progression. Thus, the aim of this study was to determine any association between the presence of microorganisms of gynecological importance, sexual behavior, clinical and demographical variables to the development and progress of cervical lesions.<h4>Methods</h4>One hundred and thirty-tw ...[more]

PMID: 29246195

Similar Datasets

Project description:BackgroundSouth African women of reproductive age have a high burden of sexually transmitted infections (STIs), including human papillomavirus (HPV) infection. However, there is limited information on the prevalence of sexually transmitted pathogens in women from rural Eastern Cape Province, South Africa. The study aims at determining the prevalence of sexually transmitted pathogens and co-infection with high-risk (HR) HPV among women from rural Eastern Cape Province, South Africa.MethodsA total of 205 cervical specimens were collected from women aged ≥ 30 years from a rural community-based clinic. The samples were tested for a panel of pathogenic STIs [Chlamydia trachomatis (serovars A-K & L1-L3), Haemophilus ducreyi, Herpes Simplex Virus (Types 1 & 2), Neisseria gonorrhoeae, Treponema pallidum, Trichomonas vaginalis (TV), and pathobionts [Mycoplasma genitalium (MG), Mycoplasma hominis (MH) and Ureaplasma spp. (UP)] using a multiplex PCR STD direct flow chip assay through a manual Hybrispot platform (Master Diagnostica, Granada, Spain). HR-HPV detection was performed by Hybrid Capture-2 assay.ResultsHigh-risk HPV prevalence was 32.2% (66/205) and HIV-1 prevalence was 38.5% (79/205). The overall prevalence of six pathogenic STIs was 22.9% (47/205), with TV having the highest prevalence (15.6%; 32/205). UP (70.2%, 144/205) and MH (36.6%, 75/205) were the most frequently detected pathobionts. Co-infection with ≥ 2 pathogens pathobionts was observed among 52.7% (108/205) participants. Of the six pathogenic STIs, three participants had more than one STI (1.46%) with the presence of MH and UP. HSV-2 (OR: 4.17, CI [1.184-14.690]) and HIV infection (OR: 2.11, CI [1.145-3.873]) were independent STIs associated with HR-HPV infection.ConclusionsThe high prevalence of pathogenic STIs underscores the need to improve syndromic management policy by implementing effective strategies of prevention, screening tests, and management. HSV-2 and HIV positive remain strongly associated with HR-HPV infection.

Dataset Information

Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil.

Background

Methods

Results

Conclusion

Publications

Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil.

Similar Datasets

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