Ontology highlight
ABSTRACT:
SUBMITTER: Ransey E
PROVIDER: S-EPMC5733776 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
FEBS letters 20170614 13
The RNA lariat debranching enzyme, Dbr1, is a metallophosphoesterase that cleaves 2'-5' phosphodiester bonds within intronic lariats. Previous reports have indicated that Dbr1 enzymatic activity is supported by diverse metal ions including Ni<sup>2+</sup> , Mn<sup>2+</sup> , Mg<sup>2+</sup> , Fe<sup>2+</sup> , and Zn<sup>2+</sup> . While in initial structures of the Entamoeba histolytica Dbr1 only one of the two catalytic metal-binding sites were observed to be occupied (with a Mn<sup>2+</sup> i ...[more]