Dataset Information

Detection of Signal Regulatory Protein ? in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody.

ABSTRACT: Non-human primates (NHP) are suitable models for studying different aspects of the human system, including pathogenesis and protective immunity to many diseases. However, the lack of specific immunological reagents for neo-tropical monkeys, such as Saimiri sciureus, is still a major factor limiting studies in these models. An alternative strategy to circumvent this obstacle has been the selection of immunological reagents directed to humans, which present cross-reactivity with NHP molecules. In this context and considering the key role of inhibitory immunoreceptors-such as the signal regulatory protein ? (SIRP?)-in the regulation of immune responses, in the present study, we attempted to evaluate the ability of anti-human SIRP? monoclonal antibodies to recognize SIRP? in antigen-presenting S. sciureus peripheral blood mononuclear cells (PBMC). As shown by flow cytometry analysis, the profile of anti-SIRP? staining as well as the levels of SIRP?-positive cells in PBMC from S. sciureus were similar to those observed in human PBMC. Furthermore, using anti-SIRP? monoclonal antibody, it was possible to detect a decrease of the SIRP? levels on surface of S. sciureus cells after in vitro stimulation with lipopolysaccharides. Finally, using computed-based analysis, we observed a high degree of conservation of SIRP? across six species of primates and the presence of shared epitopes in the extracellular domain between humans and Saimiri genus that could be targeted by antibodies. In conclusion, we have identified a commercially available anti-human monoclonal antibody that is able to detect SIRP? of S. sciureus monkeys and that, therefore, can facilitate the study of the immunomodulatory role of SIRP? when S. sciureus is used as a model.

SUBMITTER: de Souza HADS

PROVIDER: S-EPMC5735064 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Publications

Detection of Signal Regulatory Protein α in <i>Saimiri sciureus</i> (Squirrel Monkey) by Anti-Human Monoclonal Antibody.

de Souza Hugo Amorim Dos Santos HADS Costa-Correa Edmar Henrique EH Bianco-Junior Cesare C Andrade Márcia Cristina Ribeiro MCR Lima-Junior Josué da Costa JDC Pratt-Riccio Lilian Rose LR Daniel-Ribeiro Cláudio Tadeu CT Totino Paulo Renato Rivas PRR

Frontiers in immunology 20171214

Non-human primates (NHP) are suitable models for studying different aspects of the human system, including pathogenesis and protective immunity to many diseases. However, the lack of specific immunological reagents for neo-tropical monkeys, such as <i>Saimiri sciureus</i>, is still a major factor limiting studies in these models. An alternative strategy to circumvent this obstacle has been the selection of immunological reagents directed to humans, which present cross-reactivity with NHP molecul ...[more]

PMID: 29312325

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Project description:Landscape genetic studies offer a fine-scale understanding of how habitat heterogeneity influences population genetic structure. We examined population genetic structure and conducted a landscape genetic analysis for the endangered Central American Squirrel Monkey (Saimiri oerstedii) that lives in the fragmented, human-modified habitats of the Central Pacific region of Costa Rica. We analyzed non-invasively collected fecal samples from 244 individuals from 14 groups for 16 microsatellite markers. We found two geographically separate genetic clusters in the Central Pacific region with evidence of recent gene flow among them. We also found significant differentiation among groups of S. o. citrinellus using pairwise F(ST) comparisons. These groups are in fragments of secondary forest separated by unsuitable "matrix" habitats such as cattle pasture, commercial African oil palm plantations, and human residential areas. We used an individual-based landscape genetic approach to measure spatial patterns of genetic variance while taking into account landscape heterogeneity. We found that large, commercial oil palm plantations represent moderate barriers to gene flow between populations, but cattle pastures, rivers, and residential areas do not. However, the influence of oil palm plantations on genetic variance was diminished when we restricted analyses to within population pairs, suggesting that their effect is scale-dependent and manifests during longer dispersal events among populations. We show that when landscape genetic methods are applied rigorously and at the right scale, they are sensitive enough to track population processes even in species with long, overlapping generations such as primates. Thus landscape genetic approaches are extremely valuable for the conservation management of a diverse array of endangered species in heterogeneous, human-modified habitats. Our results also stress the importance of explicitly considering the heterogeneity of matrix habitats in landscape genetic studies, instead of assuming that all matrix habitats have a uniform effect on population genetic processes.

Dataset Information

Detection of Signal Regulatory Protein ? in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody.

Publications

Detection of Signal Regulatory Protein α in <i>Saimiri sciureus</i> (Squirrel Monkey) by Anti-Human Monoclonal Antibody.

Similar Datasets

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