Ontology highlight
ABSTRACT:
SUBMITTER: Chapelin F
PROVIDER: S-EPMC5735180 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Chapelin Fanny F Gao Shang S Okada Hideho H Weber Thomas G TG Messer Karen K Ahrens Eric T ET
Scientific reports 20171218 1
Discovery of effective cell therapies against cancer can be accelerated by the adaptation of tools to rapidly quantitate cell biodistribution and survival after delivery. Here, we describe the use of nuclear magnetic resonance (NMR) 'cytometry' to quantify the biodistribution of immunotherapeutic T cells in intact tissue samples. In this study, chimeric antigen receptor (CAR) T cells expressing EGFRvIII targeting transgene were labeled with a perfluorocarbon (PFC) emulsion ex vivo and infused in ...[more]