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Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells.


ABSTRACT: Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.

SUBMITTER: Oudelaar AM 

PROVIDER: S-EPMC5736099 | biostudies-literature |

REPOSITORIES: biostudies-literature

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