Ontology highlight
ABSTRACT:
SUBMITTER: Grintsevich EE
PROVIDER: S-EPMC5736627 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Grintsevich Elena E EE Ge Peng P Sawaya Michael R MR Yesilyurt Hunkar Gizem HG Terman Jonathan R JR Zhou Z Hong ZH Reisler Emil E
Nature communications 20171219 1
Actin filament assembly and disassembly are vital for cell functions. MICAL Redox enzymes are important post-translational effectors of actin that stereo-specifically oxidize actin's M44 and M47 residues to induce cellular F-actin disassembly. Here we show that Mical-oxidized (Mox) actin can undergo extremely fast (84 subunits/s) disassembly, which depends on F-actin's nucleotide-bound state. Using near-atomic resolution cryoEM reconstruction and single filament TIRF microscopy we identify two d ...[more]