Ontology highlight
ABSTRACT:
SUBMITTER: Okoniewski SR
PROVIDER: S-EPMC5737210 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Okoniewski Stephen R SR Uyetake Lyle L Perkins Thomas T TT
Nucleic acids research 20171001 18
Single-molecule force spectroscopy provides insight into how proteins bind to and move along DNA. Such studies often embed a single-stranded (ss) DNA region within a longer double-stranded (ds) DNA molecule. Yet, producing these substrates remains laborious and inefficient, particularly when using the traditional three-way hybridization. Here, we developed a force-activated substrate that yields an internal 1000 nucleotide (nt) ssDNA region when pulled partially into the overstretching transitio ...[more]