Ontology highlight
ABSTRACT:
SUBMITTER: Fujiwara K
PROVIDER: S-EPMC5737407 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Fujiwara Kei K Sawamura Tsunehito T Niwa Tatsuya T Deyama Tatsuki T Nomura Shin-Ichiro M SM Taguchi Hideki H Doi Nobuhide N
Nucleic acids research 20171101 19
In vitro transcription-translation systems (TX-TL) can synthesize most of individual genes encoded in genomes by using strong promoters and translation initiation sequences. This fact raises a possibility that TX-TL using genome as a template can reconstitute the profile of RNA and proteins in living cells. By using cell extracts and genome prepared from different organisms, here we developed a system for in vitro genome transcription-translation (iGeTT) using bacterial genome and cell extracts, ...[more]