Ontology highlight
ABSTRACT:
SUBMITTER: Olorunniji FJ
PROVIDER: S-EPMC5737554 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Olorunniji Femi J FJ McPherson Arlene L AL Rosser Susan J SJ Smith Margaret C M MCM Colloms Sean D SD Stark W Marshall WM
Nucleic acids research 20170801 14
Bacteriophage serine integrases are extensively used in biotechnology and synthetic biology for assembly and rearrangement of DNA sequences. Serine integrases promote recombination between two different DNA sites, attP and attB, to form recombinant attL and attR sites. The 'reverse' reaction requires another phage-encoded protein called the recombination directionality factor (RDF) in addition to integrase; RDF activates attL × attR recombination and inhibits attP × attB recombination. We show h ...[more]