Unknown

Dataset Information

0

A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC.


ABSTRACT: In most eukaryotes, RNA silencing is an adaptive immune system regulating key biological processes including antiviral defense. To evade this response, viruses of plants, worms and insects have evolved viral suppressors of RNA silencing proteins (VSRs). Various VSRs, such as P1 from Sweet potato mild mottle virus (SPMMV), inhibit the activity of RNA-induced silencing complexes (RISCs) including an ARGONAUTE (AGO) protein loaded with a small RNA. However, the specific mechanisms explaining this class of inhibition are unknown. Here, we show that SPMMV P1 interacts with AGO1 and AGO2 from Arabidopsis thaliana, but solely interferes with AGO1 function. Moreover, a mutational analysis of a newly identified zinc finger domain in P1 revealed that this domain could represent an effector domain as it is required for P1 suppressor activity but not for AGO1 binding. Finally, a comparative analysis of the target RNA binding capacity of AGO1 in the presence of wild-type or suppressor-defective P1 forms revealed that P1 blocks target RNA binding to AGO1. Our results describe the negative regulation of RISC, the small RNA containing molecular machine.

SUBMITTER: Kenesi E 

PROVIDER: S-EPMC5737661 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC.

Kenesi Erzsébet E   Carbonell Alberto A   Lózsa Rita R   Vértessy Beáta B   Lakatos Lóránt L  

Nucleic acids research 20170701 13


In most eukaryotes, RNA silencing is an adaptive immune system regulating key biological processes including antiviral defense. To evade this response, viruses of plants, worms and insects have evolved viral suppressors of RNA silencing proteins (VSRs). Various VSRs, such as P1 from Sweet potato mild mottle virus (SPMMV), inhibit the activity of RNA-induced silencing complexes (RISCs) including an ARGONAUTE (AGO) protein loaded with a small RNA. However, the specific mechanisms explaining this c  ...[more]

Similar Datasets

| S-EPMC3421681 | biostudies-literature
| S-EPMC2904775 | biostudies-literature
| S-EPMC1913063 | biostudies-literature
| S-EPMC4368812 | biostudies-literature
| S-EPMC8682738 | biostudies-literature
| S-EPMC4694583 | biostudies-literature
| S-EPMC5576611 | biostudies-literature
| S-EPMC2760488 | biostudies-literature
| S-EPMC1866121 | biostudies-literature
| S-EPMC3874164 | biostudies-literature