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Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus.


ABSTRACT: The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus.

SUBMITTER: Evangelista CCS 

PROVIDER: S-EPMC5738622 | biostudies-literature | 2017 Oct-Dec

REPOSITORIES: biostudies-literature

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Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus.

Evangelista Cláudia Carolina Silva CCS   Guidelli Giovanna Vieira GV   Borges Gustavo G   Araujo Thais Fenz TF   Souza Tiago Alves Jorge de TAJ   Neves Ubiraci Pereira da Costa UPDC   Tunnacliffe Alan A   Pereira Tiago Campos TC  

Genetics and molecular biology 20171001 4


The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involve  ...[more]

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