Dataset Information

Dominance of the Enterocytozoon bieneusi genotype BEB6 in red deer (Cervus elaphus) and Siberian roe deer (Capreolus pygargus) in China and a brief literature review.

ABSTRACT: Enterocytozoon?bieneusi is the most frequently diagnosed microsporidian species in humans and is also found in a wide range of animals. It is considered to be an important but neglected zoonotic pathogen. With the development of deer bred in captivity, the number of deer has been increasing in recent years in China and there are more people involved in this work. The aims of this study were to determine prevalence and genotypes of E.?bieneusi in red deer (Cervus?elaphus) and Siberian roe deer (Capreolus?pygargus), and to assess their potential zoonotic transmission. A total of 122?fecal specimens were collected from 104?red?deer and 18?roe?deer from three deer farms in Heilongjiang and Jilin Provinces, China. Enterocytozoon?bieneusi was detected and genotyped by PCR and sequencing of the internal?transcribed?spacer?(ITS) region of the rRNA gene. The average infection rate was 8.2%?(10/122), with 7.7% (8/104) for red deer and 11.1% (2/18) for roe deer. Two genotypes were identified: a known genotype BEB6 (n?=?9) and a novel genotype named HLJD-VI?(n?=?1). This is the first report of E.?bieneusi infection in Siberian roe deer. The fact that genotype BEB6 was detected previously in one human case of microsporidiosis, and that genotype HLJD-VI fell into zoonotic group?1, suggest the possibility of transmission to humans. A brief review of E.?bieneusi genotypes in deer worldwide shows that 40?genotypes have been found in seven deer species, with genotype BEB6 being predominant.

SUBMITTER: Zhao W

PROVIDER: S-EPMC5739546 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Dominance of the Enterocytozoon bieneusi genotype BEB6 in red deer (Cervus elaphus) and Siberian roe deer (Capreolus pygargus) in China and a brief literature review.

Zhao Wei W Wang Jianguang J Yang Ziyin Z Liu Aiqin A

Parasite (Paris, France) 20171221

Enterocytozoon bieneusi is the most frequently diagnosed microsporidian species in humans and is also found in a wide range of animals. It is considered to be an important but neglected zoonotic pathogen. With the development of deer bred in captivity, the number of deer has been increasing in recent years in China and there are more people involved in this work. The aims of this study were to determine prevalence and genotypes of E. bieneusi in red deer (Cervus elaphus) and Siberian roe deer (C ...[more]

PMID: 29267159

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Project description:Background. Understanding species distribution, especially areas of overlapping habitat between sympatric species, is essential for informing conservation through natural habitat protection. New protection strategies should simultaneously consider conservation efforts for multiple species that exist within the same landscape, which requires studies that include habitat overlap analysis. Methods. We estimated the potential habitat of cervids, which are typical ungulates in northern China, using the present locations of red deer (Cervus elaphus; N = 90) and roe deer (Capreolus capreolus; N = 106) in a Maximum Entropy (MaxEnt) model. Our study area was a human-dominated landscape in the Tieli Forestry Bureau located at the southern slope of the Lesser Xing'an Mountains. We grouped 17 environmental predictor variables into five predictor classes (terrain, habitat accessibility, land cover, vegetation feature, and interference), which were used to build habitat suitability models. Results. Habitat accessibility and human interferences were found to have the strongest influence on habitat suitability among the five variable classes. Among the environmental factors, distance to farmland (26.8%), distance to bush-grass land (14.6%), elevation (13.5%), and distance to water source (12.2%) were most important for red deer, distance to farmland (22.9%), distance to settlement (21.4%), elevation (11.6%), and coverage of shrub-grass (8%) were most important for roe deer. Model accuracy was high for both species (mean area under the curve (AUC) = 0.936 for red deer and 0.924 for roe deer). The overlapping habitat comprised 89.93 km(2) within the study area, which occupied 94% of potentially suitable habitat for red deer and 27% for roe deer. Conclusions. In terms of habitat suitability, roe deer showed greater selectivity than red deer. The overlapping habitat was mostly located in the eastern mountains. The southwestern plain was not a suitable habitat for deer because it was close to Tieli City. Regarding management measures, we suggest that priority protection should be given to the potential areas of overlapping deer habitats found in this study.

Project description:BackgroundMost eukaryotic species represent stable karyotypes with a particular diploid number. B chromosomes are additional to standard karyotypes and may vary in size, number and morphology even between cells of the same individual. For many years it was generally believed that B chromosomes found in some plant, animal and fungi species lacked active genes. Recently, molecular cytogenetic studies showed the presence of additional copies of protein-coding genes on B chromosomes. However, the transcriptional activity of these genes remained elusive. We studied karyotypes of the Siberian roe deer (Capreolus pygargus) that possess up to 14 B chromosomes to investigate the presence and expression of genes on supernumerary chromosomes.ResultsHere, we describe a 2 Mbp region homologous to cattle chromosome 3 and containing TNNI3K (partial), FPGT, LRRIQ3 and a large gene-sparse segment on B chromosomes of the Siberian roe deer. The presence of the copy of the autosomal region was demonstrated by B-specific cDNA analysis, PCR assisted mapping, cattle bacterial artificial chromosome (BAC) clone localization and quantitative polymerase chain reaction (qPCR). By comparative analysis of B-specific and non-B chromosomal sequences we discovered some B chromosome-specific mutations in protein-coding genes, which further enabled the detection of a FPGT-TNNI3K transcript expressed from duplicated genes located on B chromosomes in roe deer fibroblasts.ConclusionsDiscovery of a large autosomal segment in all B chromosomes of the Siberian roe deer further corroborates the view of an autosomal origin for these elements. Detection of a B-derived transcript in fibroblasts implies that the protein coding sequences located on Bs are not fully inactivated. The origin, evolution and effect on host of B chromosomal genes seem to be similar to autosomal segmental duplications, which reinforces the view that supernumerary chromosomal elements might play an important role in genome evolution.

Dataset Information

Dominance of the Enterocytozoon bieneusi genotype BEB6 in red deer (Cervus elaphus) and Siberian roe deer (Capreolus pygargus) in China and a brief literature review.

Publications

Dominance of the Enterocytozoon bieneusi genotype BEB6 in red deer (Cervus elaphus) and Siberian roe deer (Capreolus pygargus) in China and a brief literature review.

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