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SphK2 over-expression promotes osteosarcoma cell growth.

ABSTRACT: It is needed to explore novel biological markers for early diagnosis and treatment of human osteosarcoma. Sphingosine kinase 2 (SphK2) expression and potential functions in osteosarcoma were studied. We demonstrate that SphK2 is over-expressed in multiple human osteosarcoma tissues and established human osteosarcoma cell lines. Silence of SphK2 by targeted-shRNAs inhibited osteosarcoma cell growth, and induced cell apoptosis. On the other hand, exogenous over-expression of SphK2 could further promote osteosarcoma cell growth. Notably, microRNA-19a-3p ("miR-19a-3p") targets the 3' UTR (untranslated region) of SphK2 mRNA. Remarkably, forced-expression of miR-19a-3p silenced SphK2 and inhibited osteosarcoma cell growth. In vivo, SphK2 silence, by targeted-shRNA or miR-19a-3p, inhibited U2OS tumor growth in nude mice. These results suggest that SphK2 could be a novel and key oncotarget protein for OS cell progression.

SUBMITTER: Xu D

PROVIDER: S-EPMC5739656 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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SphK2 over-expression promotes osteosarcoma cell growth.

Xu Dawei D Zhu Hao H Wang Chengniu C Zhao Wei W Liu Genxiang G Bao Guofeng G Cui Daoran D Fan Jianbo J Wang Fei F Jin Huricha H Cui Zhiming Z

Oncotarget 20171106 62

It is needed to explore novel biological markers for early diagnosis and treatment of human osteosarcoma. Sphingosine kinase 2 (SphK2) expression and potential functions in osteosarcoma were studied. We demonstrate that SphK2 is over-expressed in multiple human osteosarcoma tissues and established human osteosarcoma cell lines. Silence of SphK2 by targeted-shRNAs inhibited osteosarcoma cell growth, and induced cell apoptosis. On the other hand, exogenous over-expression of SphK2 could further pr ...[more]

PMID: 29285269

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SphK2 over-expression promotes osteosarcoma cell growth.

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SphK2 over-expression promotes osteosarcoma cell growth.

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