Unknown

Dataset Information

0

Feasibility of monitoring advanced melanoma patients using cell-free DNA from plasma.


ABSTRACT: To determine the feasibility of liquid biopsy for monitoring of patients with advanced melanoma, cell-free DNA was extracted from plasma for 25 Stage III/IV patients, most (84.0%) having received previous therapy. DNA concentrations ranged from 0.6 to 390.0 ng/ml (median = 7.8 ng/ml) and were positively correlated with tumor burden as measured by imaging (Spearman rho = 0.5435, p = .0363). Using ultra-deep sequencing for a 61-gene panel, one or more mutations were detected in 12 of 25 samples (48.0%), and this proportion did not vary significantly for patients on or off therapy at the time of blood draw (52.9% and 37.5% respectively; p = .673). Sixteen mutations were detected in eight different genes, with the most frequent mutations detected in BRAF, NRAS, and KIT. Allele fractions ranged from 1.1% to 63.2% (median = 29.1%). Among patients with tissue next-generation sequencing, nine of 11 plasma mutations were also detected in matched tissue, for a concordance of 81.8%.

SUBMITTER: Gangadhar TC 

PROVIDER: S-EPMC5742050 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications


To determine the feasibility of liquid biopsy for monitoring of patients with advanced melanoma, cell-free DNA was extracted from plasma for 25 Stage III/IV patients, most (84.0%) having received previous therapy. DNA concentrations ranged from 0.6 to 390.0 ng/ml (median = 7.8 ng/ml) and were positively correlated with tumor burden as measured by imaging (Spearman rho = 0.5435, p = .0363). Using ultra-deep sequencing for a 61-gene panel, one or more mutations were detected in 12 of 25 samples (4  ...[more]

Similar Datasets

| S-EPMC8305047 | biostudies-literature
| S-EPMC5754253 | biostudies-literature
| S-EPMC7446321 | biostudies-literature
| S-EPMC8531215 | biostudies-literature
| S-EPMC6521434 | biostudies-literature
| S-EPMC3507877 | biostudies-literature
| S-EPMC10104435 | biostudies-literature
| S-EPMC9185905 | biostudies-literature
2019-03-31 | E-MTAB-7163 | biostudies-arrayexpress
2020-10-29 | E-MTAB-9678 | biostudies-arrayexpress