Iron enhances the peptidyl deformylase activity and biofilm formation in Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus plays a major role in persistent infections and many of these species form structured biofilms on different surfaces which is accompanied by changes in gene expression profiles. Further, iron supplementation plays a critical role in the regulation of several protein(s)/enzyme function, which all aid in the development of active bacterial biofilms. It is well known that for each protein, deformylation is the most crucial step in biosynthesis and is catalyzed by peptidyl deformylase (PDF). Thus, the aim of the current study is to understand the role of iron in biofilm formation and deformylase activity of PDF. Hence, the PDF gene of S. aureus ATCC12600 was PCR amplified using specific primers and sequenced, followed by cloning and expression in Escherichia coli DH5?. The deformylase activity of the purified recombinant PDF was measured in culture supplemented with/without iron where the purified rPDF showed Km of 1.3 mM and Vmax of 0.035 mM/mg/min, which was close to the native PDF (Km = 1.4 mM, Vmax = 0.030 mM/mg/min). Interestingly, the Km decreased and PDF activity increased when the culture was supplemented with iron, corroborating with qPCR results showing 100- to 150-fold more expression compared to control in S. aureus and its drug-resistant strains. Further biofilm-forming units (BU) showed an incredible increase (0.42 ± 0.005 to 6.3 ± 0.05 BU), i.e., almost 15-fold elevation in anaerobic conditions, indicating the significance of iron in S. aureus biofilms.

SUBMITTER: Swarupa V 

PROVIDER: S-EPMC5742566 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature