Dataset Information

Detection and Characterization of Hepatitis E Virus in Goats at Slaughterhouse in Tai'an Region, China.

ABSTRACT: Hepatitis E virus (HEV) is a significant pathogen of viral hepatitis and can be transmitted through fecal-oral route. Epidemiological data concerning HEV in goats, however, are relatively sparse to date. Here, the prevalence and characteristics of HEV isolated from goats at slaughterhouse were investigated in Tai'an region, China.Anti-HEV immunoglobulin G (IgG) in blood samples and HEV RNA in the liver samples were determined by using an enzyme-linked immunosorbent assay (ELISA) and a nested reverse transcription polymerase chain reaction (RT-PCR), respectively. In addition, partial nucleotide sequences of open reading frame 2 (ORF-2) of HEV isolates were analyzed.Fifty goat blood samples (46.7%, 50/120) were masculine for anti-HEV IgG. HEV RNA was detected in 2 liver samples (4.0%, 2/50) and belonged to genotype 4 subtype 4?h, with high identity (91.2-93%) with cow HEV strains detected in the same province, China.These findings demonstrated that goats may be an important reservoir for HEV and can become a major source of HEV infection in humans via food chain.

SUBMITTER: Li S

PROVIDER: S-EPMC5742876 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Detection and Characterization of Hepatitis E Virus in Goats at Slaughterhouse in Tai'an Region, China.

Li Song S Liu Mingxia M Cong Jingjing J Zhou Yufa Y Miao Zengmin Z

BioMed research international 20171212

<h4>Background</h4>Hepatitis E virus (HEV) is a significant pathogen of viral hepatitis and can be transmitted through fecal-oral route. Epidemiological data concerning HEV in goats, however, are relatively sparse to date. Here, the prevalence and characteristics of HEV isolated from goats at slaughterhouse were investigated in Tai'an region, China.<h4>Methods</h4>Anti-HEV immunoglobulin G (IgG) in blood samples and HEV RNA in the liver samples were determined by using an enzyme-linked immunosor ...[more]

PMID: 29379797

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Dataset Information

Detection and Characterization of Hepatitis E Virus in Goats at Slaughterhouse in Tai'an Region, China.

Publications

Detection and Characterization of Hepatitis E Virus in Goats at Slaughterhouse in Tai'an Region, China.

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