Ontology highlight
ABSTRACT:
SUBMITTER: Casey AK
PROVIDER: S-EPMC5743091 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Casey Amanda K AK Moehlman Andrew T AT Zhang Junmei J Servage Kelly A KA Krämer Helmut H Orth Kim K
The Journal of biological chemistry 20171031 51
Protein chaperones play a critical role in proteostasis. The activity of the major endoplasmic reticulum chaperone BiP (GRP78) is regulated by Fic-mediated AMPylation during resting states. By contrast, during times of stress, BiP is deAMPylated. Here, we show that excessive AMPylation by a constitutively active Fic<sup>E247G</sup> mutant is lethal in <i>Drosophila</i> This lethality is cell-autonomous, as directed expression of the mutant Fic<sup>E247G</sup> to the fly eye does not kill the fly ...[more]