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A Two-Dimensional Human Minilung System (Model) for Respiratory Syncytial Virus Infections.

ABSTRACT: Human respiratory syncytial virus (HRSV) is a major cause of serious pediatric respiratory diseases that lacks effective vaccine or specific therapeutics. Although our understanding about HRSV biology has dramatically increased during the last decades, the need for adequate models of HRSV infection is compelling. We have generated a two-dimensional minilung from human embryonic stem cells (hESCs). The differentiation protocol yielded at least six types of lung and airway cells, although it is biased toward the generation of distal cells. We show evidence of HRSV replication in lung cells, and the induction of innate and proinflammatory responses, thus supporting its use as a model for the study of HRSV-host interactions.

SUBMITTER: Magro-Lopez E

PROVIDER: S-EPMC5744153 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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A Two-Dimensional Human Minilung System (Model) for Respiratory Syncytial Virus Infections.

Magro-Lopez Esmeralda E Guijarro Trinidad T Martinez Isidoro I Martin-Vicente Maria M Liste Isabel I Zambrano Alberto A

Viruses 20171210 12

Human respiratory syncytial virus (HRSV) is a major cause of serious pediatric respiratory diseases that lacks effective vaccine or specific therapeutics. Although our understanding about HRSV biology has dramatically increased during the last decades, the need for adequate models of HRSV infection is compelling. We have generated a two-dimensional minilung from human embryonic stem cells (hESCs). The differentiation protocol yielded at least six types of lung and airway cells, although it is bi ...[more]

PMID: 29232863

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