Structural changes of tailless bacteriophage ?X174 during penetration of bacterial cell walls.
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ABSTRACT:
SUBMITTER: Sun Y
PROVIDER: S-EPMC5748221 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
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Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls.
Sun Yingyuan Y Roznowski Aaron P AP Tokuda Joshua M JM Klose Thomas T Mauney Alexander A Pollack Lois L Fane Bentley A BA Rossmann Michael G MG
Proceedings of the National Academy of Sciences of the United States of America 20171211 52
Unlike tailed bacteriophages, which use a preformed tail for transporting their genomes into a host bacterium, the ssDNA bacteriophage ΦX174 is tailless. Using cryo-electron microscopy and time-resolved small-angle X-ray scattering, we show that lipopolysaccharides (LPS) form bilayers that interact with ΦX174 at an icosahedral fivefold vertex and induce single-stranded (ss) DNA genome ejection. The structures of ΦX174 complexed with LPS have been determined for the pre- and post-ssDNA ejection s ...[more]
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