Ontology highlight
ABSTRACT:
SUBMITTER: Ren J
PROVIDER: S-EPMC5748491 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Ren Jihui J Snider Justin J Airola Michael V MV Zhong Aaron A Rana Nadia A NA Obeid Lina M LM Hannun Yusuf A YA
Journal of lipid research 20171101 1
Serine palmitoyltransferase (SPT) catalyzes the rate-limiting step of condensation of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine (3KDS). Here, we report a HPLC-ESI-MS/MS method to directly quantify 3KDS generated by SPT. With this technique, we were able to detect 3KDS at a level comparable to that of dihydrosphingosine in yeast <i>Saccharomyces cerevisiae</i> An in vitro SPT assay measuring the incorporation of deuterated serine into deuterated 3KDS was developed. The results s ...[more]