Ontology highlight
ABSTRACT:
SUBMITTER: Burz DS
PROVIDER: S-EPMC5748938 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Burz David S DS DeMott Christopher M CM Aldousary Asma A Dansereau Stephen S Shekhtman Alexander A
Methods in molecular biology (Clifton, N.J.) 20180101
This paper describes three protocols for identifying interacting surfaces on <sup>15</sup>N-labeled target proteins of known structure by using in-cell NMR spectroscopy. The first protocol describes how to identify protein quinary structure interaction surfaces in prokaryotes by using cross-relaxation-induced polarization transfer, CRIPT, based in-cell NMR. The second protocol describes how to introduce labeled protein into eukaryotic (HeLa) cells via electroporation for CRIPT-based in-cell stud ...[more]