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Root lodging is a physical stress that changes gene expression from sucrose accumulation to degradation in sorghum.

ABSTRACT: Sorghum (Sorghum bicolor L.) is used as a raw material for biofuels because it accumulates sugars at high levels in the stem. Lodging of sorghum occurs when the soil is wet and very high winds blow across the field. In root lodging, the roots are pulled loose from the soil, causing the plant to fall over. Lodging reduces the yield of nonstructural carbohydrates. It is not yet clear which genes show changes in expression when sorghum falls over. We compared whole-gene expression in the mature stems of intact and lodged sorghum plants, with a focus on comparisons from the perspective of differences in sugar accumulation or degradation.Lodging decreased sucrose content, starch content, and ratio of sucrose to total sugars in the stems of the sorghum cultivar SIL-05. Particular paralogs of SWEET and TMT family genes, which encode sucrose or hexose transporters, or both, were significantly highly expressed in intact or lodged sorghum stems. In intact stems, genes encoding the glucose-6-phosphate translocator, aquaporins, and enzymes involved in photosynthesis and starch synthesis were highly expressed. In lodged sorghum stems, expression of genes associated with sucrose or starch degradation or energy production was increased. Notably, expression of genes encoding enzymes catalyzing irreversible reactions and associated with the first steps of these metabolic pathways (e.g. INV, SUS, and hexokinase- and fructokinase-encoding genes) was significantly increased by lodging. Expression of SUT, SPS, and SPP was almost the same in intact and lodged sorghum.Specific paralogs of sucrose-associated genes involved in metabolic pathways and in membrane transport were expressed in the stems of sorghum SIL-05 at the full-ripe stage. Root lodging drastically changed the expression of these genes from sucrose accumulation to degradation. The changes in gene expression resulted in decreases in sugar content and in the proportion of sucrose to hexoses in the stems of lodged plants.

SUBMITTER: Mizuno H

PROVIDER: S-EPMC5751775 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Root lodging is a physical stress that changes gene expression from sucrose accumulation to degradation in sorghum.

Mizuno Hiroshi H Kasuga Shigemitsu S Kawahigashi Hiroyuki H

BMC plant biology 20180103 1

<h4>Background</h4>Sorghum (Sorghum bicolor L.) is used as a raw material for biofuels because it accumulates sugars at high levels in the stem. Lodging of sorghum occurs when the soil is wet and very high winds blow across the field. In root lodging, the roots are pulled loose from the soil, causing the plant to fall over. Lodging reduces the yield of nonstructural carbohydrates. It is not yet clear which genes show changes in expression when sorghum falls over. We compared whole-gene expressio ...[more]

PMID: 29298675

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Project description:BackgroundSorghum is an important food staple in the developing world, with the capacity to grow under severe conditions such as salinity, drought, and a limited nutrient supply. As a serious environmental stress, soil salinization can change the composition of rhizosphere soil bacterial communities and induce a series of harm to crops. And the change of rhizospheric microbes play an important role in the response of plants to salt stress. However, the effect of salt stress on the root bacteria of sorghum and interactions between bacteria and sorghum remains poorly understood.ResultsThe purpose of this study was to assess the effect of salt stress on sorghum growth performance and rhizosphere bacterial community structure. Statistical analysis confirmed that low high concentration stress depressed sorghum growth. Further taxonomic analysis revealed that the bacterial community predominantly consisted of phyla Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, Bacteroidetes and Firmicutes in sorghum rhizosphere soil. Low salt stress suppressed the development of bacterial diversity less than high salt stress in both bulk soil and planted sorghum soil. Different sorghum development stages in soils with different salt concentrations enriched distinctly different members of the root bacteria. No obviously different effect on bacterial diversity were tested by PERMANOVA analysis between different varieties, but interactions between salt and growth and between salt and variety were detected. The roots of sorghum exuded phenolic compounds that differed among the different varieties and had a significant relationship with rhizospheric bacterial diversity. These results demonstrated that salt and sorghum planting play important roles in restructuring the bacteria in rhizospheric soil. Salinity and sorghum variety interacted to affect bacterial diversity.ConclusionsIn this paper, we found that salt variability and planting are key factors in shifting bacterial diversity and community. In comparison to bulk soils, soils under planting sorghum with different salt stress levels had a characteristic bacterial environment. Salinity and sorghum variety interacted to affect bacterial diversity. Different sorghum variety with different salt tolerance levels had different responses to salt stress by regulating root exudation. Soil bacterial community responses to salinity and exotic plants could potentially impact the microenvironment to help plants overcome external stressors and promote sorghum growth. While this study observed bacterial responses to combined effects of salt and sorghum development, future studies are needed to understand the interaction among bacteria communities, salinity, and sorghum growth.

Project description:BackgroundSugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high biomass, shown great potential for biofuel, has lower sugar content than sugarcane. To enhance sugar content, the sucrose isomerase (SI) gene, driven by stem-specific promoters (A2 or LSG) with a vacuole-targetted signal peptide, was transformed into the sorghum inbred line (T×430).ResultsThe study demonstrated that transgenic lines of grain sorghum, containing 50-60% isomaltulose, accumulated up to eightfold (1000 mM) more total sugar than the control T×430 did (118 mM) in stalks of T0 generation. Subsequently, the elite engineered lines (A5, and LSG9) were crossed with sweet sorghum (Rio, and R9188). Total sugar contents (over 750 mM), were notably higher in F1, and F2 progenies than the control Rio (480 mM). The sugar contents of the engineered lines (over 750 mM), including T0, T1, F1, and F2, are surprisingly higher than that of the field-grown sugarcane (normal range 600-700 mmol/L). Additionally, analysis of physiological characterization demonstrated that the superior progenies had notably higher rates of photosynthesis, sucrose transportation, and sink strength than the controls.ConclusionsThe genetic engineering approach has dramatically enhanced total sugar content in grain sorghum (T0, and T1) and hybrid sorghum (F1, and F2), demonstrating that sorghum can accumulate as high or higher sugar content than sugarcane. This research illustrates that the SI gene has enormous potential on improvement of sugar content in sorghum, particularly in hybirds and sweet sorghum. The substantial increase on sugar content would lead to significant financial benefits for industrial utilization. This study could have a substantial impact on renewable bioenergy. More importantly, our results demonstrated that the phenotype of high sugar content is inheritable and shed light on improvement for other sugar crops.

Dataset Information

Root lodging is a physical stress that changes gene expression from sucrose accumulation to degradation in sorghum.

Publications

Root lodging is a physical stress that changes gene expression from sucrose accumulation to degradation in sorghum.

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