Dataset Information

Optimization of Extract Method for Cynomorium songaricum Rupr. by Response Surface Methodology.

ABSTRACT: The present study aimed to evaluate the quality of Cynomorium songaricum Rupr. from different producing areas, which is an edible, holoparasitic, and desert plant that has been used in traditional medicine for improving immunity and kidney function and treating constipation. We optimized the extract conditions by response surface methodology (RSM) and determined the content of gallic acid, protocatechuic acid, and catechin of C. songaricum simultaneously from different producing areas by high-performance liquid chromatography (HPLC). It is the first study in which the RSM was used to optimize the extract condition of C. songaricum with multiple evaluation factors, ensuring the efficiency and accuracy of the study. The results were analyzed by principal component analysis, and they showed that the quality of C. songaricum from Qinghai Province was the best, while the quality of C. songaricum from Gansu Province was the worst. For the first time, the two ecotypes of C. songaricum, including Outside Great Wall type and Inside Great Wall type, were discovered and identified by the chemical marker protocatechuic acid. This study is the scientific basis for quality evaluation, especially for food safety.

SUBMITTER: Zhang X

PROVIDER: S-EPMC5757168 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Optimization of Extract Method for Cynomorium songaricum Rupr. by Response Surface Methodology.

Zhang Xiang X Gu Caimei C Ahmad Bashir B Huang Linfang L

Journal of analytical methods in chemistry 20171224

The present study aimed to evaluate the quality of Cynomorium songaricum Rupr. from different producing areas, which is an edible, holoparasitic, and desert plant that has been used in traditional medicine for improving immunity and kidney function and treating constipation. We optimized the extract conditions by response surface methodology (RSM) and determined the content of gallic acid, protocatechuic acid, and catechin of C. songaricum simultaneously from different producing ar ...[more]

PMID: 29435384

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Project description:Centella asiatica (L.) Urban extracts are widely used as food, drugs and cosmetics, and the main active compounds are glycosides (madecassoside and asiaticoside) and aglycones (madecassic acid and asiatic acid). Green extraction is an interesting concept that can produce safe and high-quality extracts that use less solvent, time and energy with the environmental friendly. This study investigated the optimum conditions for extracting a triterpenoid glycoside-enriched C. asiatica extract using microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE). Central composite design and response surface methodology (RSM) were used for the experimental design and data analysis. Four-month-old C. asiatica tetraploid plants were selected as the elite raw material containing high amount of triterpenoid glycosides for the extraction experiments, and the triterpenoid content was determined by a validated HPLC method. The results demonstrated that the RSM models and equations were reliable and could predict the optimal conditions to enhance C. asiatica extract yield, glycoside and aglycone amounts. The percent of ethanol was the major factor that had a significant effect on C. asiatica yield and glycoside and aglycone content during MAE and UAE. The maximum triterpenoids content in extract; 7.332 ± 0.386% w/w madecassoside and 4.560 ± 0.153% w/w asiaticoside 0.357 ± 0.013% w/w madecassic acid and 0.209 ± 0.025% w/w asiatic acid were obtained by MAE with 80% ethanol at 100 watts for 7.5 min, whereas the optimal conditions for highest total triterpenoids extraction from dry plant was UAE with 80% ethanol, temperature 48 °C, 50 min enhanced 2.262 ± 0.046% w/w madecassoside, 1.325 ± 0.062% w/w asiaticoside, 0.082 ± 0.009% w/w madecassic acid and 0.052 ± 0.007% w/w asiatic acid as secondary outcome. Moreover, it was found that MAE and UAE consumed energy 59 and 54%, respectively, lower than that of the conventional method, maceration, in term of kilowatt-hour per gram of total triterpenoids. These optimized green conditions could be recommended for C. asiatica extraction for triterpenoid glycoside-enriched extracts production for the pharmaceutical or cosmeceutical industries and triterpenoids quantitative analysis in raw materials.

Dataset Information

Optimization of Extract Method for Cynomorium songaricum Rupr. by Response Surface Methodology.

Publications

Optimization of Extract Method for <i>Cynomorium songaricum</i> Rupr. by Response Surface Methodology.

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