Introduction of H2C2-type zinc-binding residues into HIV-2 Vpr increases its expression level.
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ABSTRACT:
SUBMITTER: Koga R
PROVIDER: S-EPMC5757179 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
Introduction of H2C2-type zinc-binding residues into HIV-2 Vpr increases its expression level.
Koga Ryoko R Yamamoto Minami M Ciftci Halil Ibrahim HI Otsuka Masami M Fujita Mikako M
FEBS open bio 20171219 1
Human immunodeficiency virus type 2 has two structurally similar proteins, Vpx and Vpr. Vpx degrades the host anti-viral protein SAMHD1 and is expressed at high levels, while Vpr is responsible for cell cycle arrest and is expressed at much lower levels. We constructed a Vpr mutant with a high level of expression by replacing the amino acids HHCR/HHCH with a putative H2C2-type zinc-binding site that is carried by Vpx. Our finding suggests that during the evolution of Vpr and Vpx, zinc-binding li ...[more]
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2019-05-01 | PXD013221 | Pride