Towards high throughput GPCR crystallography: In Meso soaking of Adenosine A2A Receptor crystals.
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ABSTRACT:
SUBMITTER: Rucktooa P
PROVIDER: S-EPMC5758569 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
Towards high throughput GPCR crystallography: In Meso soaking of Adenosine A<sub>2A</sub> Receptor crystals.
Rucktooa Prakash P Cheng Robert K Y RKY Segala Elena E Geng Tian T Errey James C JC Brown Giles A GA Cooke Robert M RM Marshall Fiona H FH Doré Andrew S AS
Scientific reports 20180108 1
Here we report an efficient method to generate multiple co-structures of the A<sub>2A</sub> G protein-coupled receptor (GPCR) with small-molecules from a single preparation of a thermostabilised receptor crystallised in Lipidic Cubic Phase (LCP). Receptor crystallisation is achieved following purification using a low affinity "carrier" ligand (theophylline) and crystals are then soaked in solutions containing the desired (higher affinity) compounds. Complete datasets to high resolution can then ...[more]
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