Dataset Information

Interactive Effects of CO2 Concentration and Water Regime on Stable Isotope Signatures, Nitrogen Assimilation and Growth in Sweet Pepper.

ABSTRACT: Sweet pepper is among the most widely cultivated horticultural crops in the Mediterranean basin, being frequently grown hydroponically under cover in combination with CO2 fertilization and water conditions ranging from optimal to suboptimal. The aim of this study is to develop a simple model, based on the analysis of plant stable isotopes in their natural abundance, gas exchange traits and N concentration, to assess sweet pepper growth. Plants were grown in a growth chamber for near 6 weeks. Two [CO2] (400 and 800 ?mol mol-1), three water regimes (control and mild and moderate water stress) and four genotypes were assayed. For each combination of genotype, [CO2] and water regime five plants were evaluated. Water stress applied caused significant decreases in water potential, net assimilation, stomatal conductance, intercellular to atmospheric [CO2], and significant increases in water use efficiency, leaf chlorophyll content and carbon isotope composition, while the relative water content, the osmotic potential and the content of anthocyanins did change not under stress compared to control conditions support this statement. Nevertheless, water regime affects plant growth via nitrogen assimilation, which is associated with the transpiration stream, particularly at high [CO2], while the lower N concentration caused by rising [CO2] is not associated with stomatal closure. The stable isotope composition of carbon, oxygen, and nitrogen (?13C, ?18O, and ?15N) in plant matter are affected not only by water regime but also by rising [CO2]. Thus, ?18O increased probably as response to decreases in transpiration, while the increase in ?15N may reflect not only a lower stomatal conductance but a higher nitrogen demand in leaves or shifts in nitrogen metabolism associated with decreases in photorespiration. The way that ?13C explains differences in plant growth across water regimes within a given [CO2], seems to be mediated through its direct relationship with N accumulation in leaves. The changes in the profile and amount of amino acids caused by water stress and high [CO2] support this conclusion. However, the results do not support the use of ?18O as an indicator of the effect of water regime on plant growth.

SUBMITTER: Serret MD

PROVIDER: S-EPMC5758588 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Interactive Effects of CO<sub>2</sub> Concentration and Water Regime on Stable Isotope Signatures, Nitrogen Assimilation and Growth in Sweet Pepper.

Serret María D MD Yousfi Salima S Vicente Rubén R Piñero María C MC Otálora-Alcón Ginés G Del Amor Francisco M FM Araus José L JL

Frontiers in plant science 20180104

Sweet pepper is among the most widely cultivated horticultural crops in the Mediterranean basin, being frequently grown hydroponically under cover in combination with CO<sub>2</sub> fertilization and water conditions ranging from optimal to suboptimal. The aim of this study is to develop a simple model, based on the analysis of plant stable isotopes in their natural abundance, gas exchange traits and N concentration, to assess sweet pepper growth. Plants were grown in a growth chamber for near 6 ...[more]

PMID: 29354140

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Project description:Background and aimsMycoheterotrophy entails plants meeting all or a portion of their carbon (C) demands via symbiotic interactions with root-inhabiting mycorrhizal fungi. Ecophysiological traits of mycoheterotrophs, such as their C stable isotope abundances, strongly correlate with the degree of species' dependency on fungal C gains relative to C gains via photosynthesis. Less explored is the relationship between plant evolutionary history and mycoheterotrophic plant ecophysiology. We hypothesized that the C and nitrogen (N) stable isotope compositions, and N concentrations of fully and partially mycoheterotrophic species differentiate them from autotrophs, and that plant family identity would be an additional and significant explanatory factor for differences in these traits among species. We focused on mycoheterotrophic species that associate with ectomycorrhizal fungi from plant families Ericaceae and Orchidaceae.MethodsPublished and unpublished data were compiled on the N concentrations, C and N stable isotope abundances (δ(13)C and δ(15)N) of fully (n = 18) and partially (n = 22) mycoheterotrophic species from each plant family as well as corresponding autotrophic reference species (n = 156). These data were used to calculate site-independent C and N stable isotope enrichment factors (ε). Then we tested for differences in N concentration, (13)C and (15)N enrichment among plant families and trophic strategies.Key resultsWe found that in addition to differentiating partially and fully mycoheterotrophic species from each other and from autotrophs, C and N stable isotope enrichment also differentiates plant species based on familial identity. Differences in N concentrations clustered at the plant family level rather than the degree of dependency on mycoheterotrophy.ConclusionsWe posit that differences in stable isotope composition and N concentrations are related to plant family-specific physiological interactions with fungi and their environments.

Project description:Intensive vegetable production is commonly associated with excessive nitrogen (N) fertilization and associated environmental problems. Monitoring of crop N status can enhance crop N management. Chlorophyll meters (CMs) could be used to monitor crop N status because leaf chlorophyll (chl) content is strongly related to crop N status. To monitor crop N status, relationships between CM measurements and leaf chl content require evaluation, particularly when excessive N is supplied. The SPAD-502 meter, atLEAF+ sensor, MC-100 Chlorophyll Concentration Meter, and Multiplex sensor were evaluated in sweet pepper with different N supply, throughout the crop, ranging from very deficient to very excessive. CM measurements of all sensors and indices were strongly and positively related to leaf chlorophyll a + b content with curvilinear relationships over the entire range of chl measured (?0-80 ?g cm-2). Measurements with the SPAD-502, and atLEAF+, and of the Multiplex's simple fluorescence ratio index (SFR) had asymptotic responses to increasing leaf chl. In contrast, the MC-100's chlorophyll content index (CCI) had a progressively increasing response. At higher chlorophyll a + b contents (e.g., >40 ?g cm-2), SPAD-502, atLEAF+ and SFR measurements tended to saturate, which did not occur with CCI. Leaf chl content was most accurately estimated by CCI (R 2 = 0.87), followed by the SPAD-502 meter (R 2 = 0.85). The atLEAF+ sensor was the least accurate (R 2 = 0.76). For leaf chl estimation, CCI measured with the MC-100 meter was the most effective of the four sensors examined because it: (1) most accurately estimated leaf chl content, and (2) had no saturation response at higher leaf chl content. For non-saturating leaf chl content (?0-40 ?g cm-2), all indices were sensitive indicators. As excessive applications of N are frequent in intensive vegetable crop production, the capacity of measuring high leaf chl contents without a saturation response is an important consideration for the practical use of chlorophyll meters.

Dataset Information

Interactive Effects of CO2 Concentration and Water Regime on Stable Isotope Signatures, Nitrogen Assimilation and Growth in Sweet Pepper.

Publications

Interactive Effects of CO<sub>2</sub> Concentration and Water Regime on Stable Isotope Signatures, Nitrogen Assimilation and Growth in Sweet Pepper.

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