Ontology highlight
ABSTRACT:
SUBMITTER: Zeng Y
PROVIDER: S-EPMC5758904 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Zeng Yan Y Cui Yang Y Zhang Yong Y Zhang Yanruo Y Liang Meng M Chen Hui H Lan Jie J Song Guangtao G Lou Jizhong J
Nucleic acids research 20180101 1
CRISPR-Cas9 system has been widely used for efficient genome editing. Although the structures of Cas9 protein in complex with single-guided RNA (sgRNA) and target DNA have been resolved, the molecular details about the formation of Cas9 endonuclease R-loop structure remain elusive. Here we examine the DNA cleavage activities of Streptococcus pyogenes Cas9 (SpyCas9) and its mutants using various target sequences and study the conformational dynamics of R-loop structure during target binding using ...[more]