Unknown

Dataset Information

0

Fast and accurate identification of cryptic and sympatric mayfly species of the Baetis rhodani group.


ABSTRACT: OBJECTIVE:Species of the Baetis rhodani group are among the most widespread mayflies of the Palearctic region. However, frequent occurrence of morphologically cryptic species complicates the identification of sympatric species. Here, we proposed and tested a method for the fast, accurate, and cost-effective assignment of a large number of individuals to their putative species, based on high resolution melting profiles of a standard mitochondrial gene fragment. We tested this method using a system of three recently identified cryptic species inhabiting the Tyrrhenian Islands (western Mediterranean basin). RESULTS:Highly species-specific high resolution melting profiles were obtained, allowing the unequivocal attribution of each individual to the respective species. This assay provides a convenient and easily customizable alternative to traditional barcoding approaches, provided that the mayfly taxa occurring within the geographic area of interest have been previously identified and their high resolution melting profiles assessed.

SUBMITTER: Bisconti R 

PROVIDER: S-EPMC5759225 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Fast and accurate identification of cryptic and sympatric mayfly species of the Baetis rhodani group.

Bisconti Roberta R   Tenchini Roberta R   Belfiore Carlo C   Nascetti Giuseppe G   Canestrelli Daniele D  

BMC research notes 20180108 1


<h4>Objective</h4>Species of the Baetis rhodani group are among the most widespread mayflies of the Palearctic region. However, frequent occurrence of morphologically cryptic species complicates the identification of sympatric species. Here, we proposed and tested a method for the fast, accurate, and cost-effective assignment of a large number of individuals to their putative species, based on high resolution melting profiles of a standard mitochondrial gene fragment. We tested this method using  ...[more]

Similar Datasets

| S-EPMC6093170 | biostudies-literature
| S-EPMC4828801 | biostudies-literature
| S-EPMC5629031 | biostudies-literature
| S-EPMC5242256 | biostudies-literature
| S-EPMC4730923 | biostudies-literature
| S-EPMC3816585 | biostudies-literature
| S-EPMC5457575 | biostudies-literature
| S-EPMC8016488 | biostudies-literature
| S-EPMC4972348 | biostudies-literature
| S-EPMC3688812 | biostudies-other