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Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.

ABSTRACT: We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.

SUBMITTER: Kawasaki F

PROVIDER: S-EPMC5759927 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.

Kawasaki F F Murat P P Li Z Z Santner T T Balasubramanian S S

Chemical communications (Cambridge, England) 20170101 8

We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures. ...[more]

PMID: 28074944

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