Chemical biology of genomic DNA: minimizing PCR bias.
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ABSTRACT:
SUBMITTER: McInroy GR
PROVIDER: S-EPMC5759930 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Chemical biology of genomic DNA: minimizing PCR bias.
McInroy Gordon R GR Raiber Eun-Ang EA Balasubramanian Shankar S
Chemical communications (Cambridge, England) 20141001 81
The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe. ...[more]
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