Dataset Information

Early arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness in an animal model of arthritis.

ABSTRACT:

Introduction

Arthritis induces joint erosions and skeletal bone fragility.

Objectives

The main goal of this work was to analyze the early arthritis induced events at bone architecture and mechanical properties at tissue level.

Methods

Eighty-eight Wistar rats were randomly housed in experimental groups, as follows: adjuvant induced arthritis (AIA) (N = 47) and a control healthy group (N = 41). Rats were monitored during 22 days for the inflammatory score, ankle perimeter and body weight and sacrificed at different time points (11 and 22 days post disease induction). Bone samples were collected for histology, micro computed tomography (micro-CT), 3-point bending and nanoindentation. Blood samples were also collected for bone turnover markers and systemic cytokine quantification.

Results

At bone tissue level, measured by nanoindentation, there was a reduction of hardness in the arthritic group, associated with an increase of the ratio of bone concentric to parallel lamellae and of the area of the osteocyte lacuna. In addition, increased bone turnover and changes in the microstructure and mechanical properties were observed in arthritic animals, since the early phase of arthritis, when compared with healthy controls.

Conclusion

We have shown in an AIA rat model that arthritis induces very early changes at bone turnover, structural degradation and mechanical weakness. Bone tissue level is also affected since the early phase of arthritis, characterized by decreased tissue hardness associated with changes in bone lamella organization and osteocyte lacuna surface. These observations highlight the pertinence of immediate control of inflammation in the initial stages of arthritis.

SUBMITTER: Vidal B

PROVIDER: S-EPMC5760022 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Early arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness in an animal model of arthritis.

Vidal Bruno B Cascão Rita R Finnilä Mikko A J MAJ Lopes Inês P IP Saarakkala Simo S Zioupos Peter P Canhão Helena H Fonseca João E JE

PloS one 20180109 1

<h4>Introduction</h4>Arthritis induces joint erosions and skeletal bone fragility.<h4>Objectives</h4>The main goal of this work was to analyze the early arthritis induced events at bone architecture and mechanical properties at tissue level.<h4>Methods</h4>Eighty-eight Wistar rats were randomly housed in experimental groups, as follows: adjuvant induced arthritis (AIA) (N = 47) and a control healthy group (N = 41). Rats were monitored during 22 days for the inflammatory score, ankle perimeter an ...[more]

PMID: 29315314

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Dataset Information

Early arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness in an animal model of arthritis.

Introduction

Objectives

Methods

Results

Conclusion

Publications

Early arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness in an animal model of arthritis.

Similar Datasets

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