Ontology highlight
ABSTRACT:
SUBMITTER: Lafuente-Barquero J
PROVIDER: S-EPMC5760084 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Lafuente-Barquero Juan J Luke-Glaser Sarah S Graf Marco M Silva Sonia S Gómez-González Belén B Lockhart Arianna A Lisby Michael M Aguilera Andrés A Luke Brian B
PLoS genetics 20171227 12
RNA-DNA hybrids are naturally occurring obstacles that must be overcome by the DNA replication machinery. In the absence of RNase H enzymes, RNA-DNA hybrids accumulate, resulting in replication stress, DNA damage and compromised genomic integrity. We demonstrate that Mph1, the yeast homolog of Fanconi anemia protein M (FANCM), is required for cell viability in the absence of RNase H enzymes. The integrity of the Mph1 helicase domain is crucial to prevent the accumulation of RNA-DNA hybrids and R ...[more]