Comparative analysis of miRNA expression profiles in transgenic and non-transgenic rice using miRNA-Seq.
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ABSTRACT:
SUBMITTER: Peng C
PROVIDER: S-EPMC5762784 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
Comparative analysis of miRNA expression profiles in transgenic and non-transgenic rice using miRNA-Seq.
Peng Cheng C Chen Xiaoyun X Wang Xiaofu X Xu Xiaoli X Wei Wei W Wang Congmao C Xu Junfeng J
Scientific reports 20180110 1
Safety assessment for genetically modified organisms (GMOs) is required before their release. To date, miRNAs that play important roles in eukaryotic gene regulation have not been considered in the current assessment system. In this study, we identified 6 independent Bt and EPSPS GM rice lines using PCR and immune strip. We analyzed the expression levels of Cry1Ac and EPSPS using quantitative real-time PCR and western blot. Further, miRNAs from the developing seeds of the 6 GM rice lines and the ...[more]
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