Differential proteomics of lesional vs. non-lesional biopsies revealed non-immune mechanisms of alopecia areata.

ABSTRACT: Alopecia areata (AA) is one of the common hair disorders for which treatment is frequently ineffective and associated with relapsing episodes. Better understanding of disease mechanisms and novel therapeutic targets are thus required. From 10 AA patients, quantitative proteomics using LTQ-Orbitrap-XL mass spectrometer revealed 104 down-regulated, 4 absent, 3 up-regulated and 11 newly present proteins in lesional vs. non-lesional biopsies. Among these, the decreased levels of α-tubulin, vimentin, heat shock protein 70 (HSP70), HSP90, annexin A2 and α-enolase were successfully confirmed by Western blotting. Protein-protein interactions network analysis using STRING tool revealed that the most frequent biological processes/networks of the down-regulated proteins included tissue development, c

SUBMITTER: Thanomkitti K 

PROVIDER: S-EPMC5765109 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature