Aberrant expression and DNA methylation of lipid metabolism genes in PCOS: a new insight into its pathogenesis.

ABSTRACT: Background:Polycystic ovary syndrome (PCOS), whose etiology remains uncertain, is a highly heterogenous and genetically complex endocrine disorder. The aim of this study was to identify differentially expressed genes (DEGs) in granulosa cells (GCs) from PCOS patients and make epigenetic insights into the pathogenesis of PCOS. Results:Included in this study were 110 women with PCOS and 119 women with normal ovulatory cycles undergoing in vitro fertilization acting as the control group. RNA-seq identified 92 DEGs unique to PCOS GCs in comparison with the control group. Bioinformatic analysis indicated that synthesis of lipids and steroids was activated in PCOS GCs. 5-Methylcytosine analysis demonstrated that there was an approximate 25% reduction in global DNA methylation of GCs in PCOS women (4.44?±?0.65%) compared with the controls (6.07?±?0.72%; P?

SUBMITTER: Pan JX 

PROVIDER: S-EPMC5767000 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature