Ontology highlight
ABSTRACT:
SUBMITTER: Tomasini MD
PROVIDER: S-EPMC5768683 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Tomasini Michael D MD Wang Yingjie Y Karamafrooz Adak A Li Geoffrey G Beuming Thijs T Gao Jiali J Taylor Susan S SS Veglia Gianluigi G Simon Sanford M SM
Scientific reports 20180115 1
In fibrolamellar hepatocellular carcinoma a single genetic deletion results in the fusion of the first exon of the heat shock protein 40, DNAJB1, which encodes the J domain, with exons 2-10 of the catalytic subunit of protein kinase A, PRKACA. This produces an enzymatically active chimeric protein J-PKAcα. We used molecular dynamics simulations and NMR to analyze the conformational landscape of native and chimeric kinase, and found an ensemble of conformations. These ranged from having the J-dom ...[more]