Intelligent image-based in situ single-cell isolation.
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ABSTRACT:
SUBMITTER: Brasko C
PROVIDER: S-EPMC5768687 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
Intelligent image-based in situ single-cell isolation.
Brasko Csilla C Smith Kevin K Molnar Csaba C Farago Nora N Hegedus Lili L Balind Arpad A Balassa Tamas T Szkalisity Abel A Sukosd Farkas F Kocsis Katalin K Balint Balazs B Paavolainen Lassi L Enyedi Marton Z MZ Nagy Istvan I Puskas Laszlo G LG Haracska Lajos L Tamas Gabor G Horvath Peter P
Nature communications 20180115 1
Quantifying heterogeneities within cell populations is important for many fields including cancer research and neurobiology; however, techniques to isolate individual cells are limited. Here, we describe a high-throughput, non-disruptive, and cost-effective isolation method that is capable of capturing individually targeted cells using widely available techniques. Using high-resolution microscopy, laser microcapture microscopy, image analysis, and machine learning, our technology enables scalabl ...[more]
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