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Relationship of vitamin D receptor gene polymorphisms in Helicobacter pylori gastric patients.

ABSTRACT: Purpose:The present study aimed to investigate the association between the human VDR gene and Helicobacter pylori infection. Patients and methods:A cross-sectional study was conducted on 208 adult patients with upper gastrointestinal symptoms. Gastric biopsy specimens were obtained from each patient for molecular DNA and histological examination. Patients were genotyped for VDR gene polymorphisms using polymerase chain reaction and restriction fragment length polymorphism analysis. Results:The allelic and genotypic distribution analyses of the FokI, ApaI and TaqI polymorphisms of the VDR gene did not show distribution differences between H. pylori-positive and -negative groups. The genotype distribution observed for polymorphism BsmI deviated significantly from what was expected in a Hardy-Weinberg equilibrium test in the H. pylori-positive group (?2=29.048, p<0.001). The distribution of BsmI genotypes differed significantly between the H. pylori-negative and H. pylori-positive groups (p=0.0034), where the frequency of the bb genotype increased among H. pylori-positive individuals compared with those without infection (63.25% versus 50.55%, respectively). Conversely, the H. pylori-negative group showed a Bb frequency that was 20.27% higher than in the infected group. Conclusion:We identified a possible association between the BsmI polymorphism and infection by H. pylori. However, further research is required to clarify this relationship.

SUBMITTER: Martins DJ

PROVIDER: S-EPMC5769596 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Relationship of vitamin D receptor gene polymorphisms in <i>Helicobacter pylori</i> gastric patients.

Martins Dinelma de Jesus DJ Matos Gyselly Cb GC Loiola Rosane Sp RS D'Annibale Vivian V Corvelo Tereza T

Clinical and experimental gastroenterology 20180112

<h4>Purpose</h4>The present study aimed to investigate the association between the human <i>VDR</i> gene and <i>Helicobacter pylori</i> infection.<h4>Patients and methods</h4>A cross-sectional study was conducted on 208 adult patients with upper gastrointestinal symptoms. Gastric biopsy specimens were obtained from each patient for molecular DNA and histological examination. Patients were genotyped for <i>VDR</i> gene polymorphisms using polymerase chain reaction and restriction fragment length ...[more]

PMID: 29391820

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Project description:Background: Penicillins inhibit cell wall synthesis; therefore, H. pylori must be dividing for these antibiotics to be effective. Identifying growth responses to varying medium pH may allow design of more effective treatment regimens. Aim: To determine the effect of acidity on bacterial growth and the bactericidal efficacy of ampicillin. Methods: H. pylori were incubated in dialysis chambers suspended in 1.5L of media at various pHs with 5mM urea, with or without ampicillin, for 4, 8 or 16 hours, thus mimicking unbuffered gastric juice. Changes in gene expression, viability and survival were determined. The bacterial load of H. pylori infected gerbils was determined with and without profound acid inhibition. Results: At pH 3.0, but not at pH 4.5 or 7.4, there was decreased expression of ~400 genes, including many cell envelope biosynthesis, cell division genes and penicillin-binding proteins. In the presence of ampicillin, viability and survival declined at pH 4.5 and 7.4 but not at pH 3.0. Profound acid inhibition of H. pylori infected gerbils increased the antral bacterial load >3 fold, showing that elevation of intragastric pH stimulated growth. Conclusions: Ampicillin is bactericidal at pH 4.5 and 7.4, but not at pH 3.0, due to decreased expression of cell envelope and division genes at pH 3.0. Therefore, at pH 3.0, the pH at the gastric surface, the bacteria are non-dividing and persist with ampicillin treatment. A more effective inhibitor of acid secretion that maintains gastric pH near neutrality for 24 hours/day should enhance the efficacy of triple therapy and of amoxicillin, even allowing dual therapy for H. pylori eradication. There were a total of three biological replicates, each containing one control (incubated at pH 7.4) and one acidic (pH 3.0) sample, whose RNA were extracted on different dates. Microarray studies performed on these six samples were done in triplicate. One of the triplicates for two of the biological control triplicates were removed from the data set due to poor hybridization

Dataset Information

Relationship of vitamin D receptor gene polymorphisms in Helicobacter pylori gastric patients.

Publications

Relationship of vitamin D receptor gene polymorphisms in <i>Helicobacter pylori</i> gastric patients.

Similar Datasets

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