Ontology highlight
ABSTRACT:
SUBMITTER: Tewary M
PROVIDER: S-EPMC5769627 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Tewary Mukul M Ostblom Joel J Prochazka Laura L Zulueta-Coarasa Teresa T Shakiba Nika N Fernandez-Gonzalez Rodrigo R Zandstra Peter W PW
Development (Cambridge, England) 20170904 23
How position-dependent cell fate acquisition occurs during embryogenesis is a central question in developmental biology. To study this process, we developed a defined, high-throughput assay to induce peri-gastrulation-associated patterning in geometrically confined human pluripotent stem cell (hPSC) colonies. We observed that, upon BMP4 treatment, phosphorylated SMAD1 (pSMAD1) activity in the colonies organized into a radial gradient. We developed a reaction-diffusion (RD)-based computational mo ...[more]