Dataset Information

Characterizing ZC3H18, a Multi-domain Protein at the Interface of RNA Production and Destruction Decisions.

ABSTRACT: Nuclear RNA metabolism is influenced by protein complexes connecting to both RNA-productive and -destructive pathways. The ZC3H18 protein binds the cap-binding complex (CBC), universally present on capped RNAs, while also associating with the nuclear exosome targeting (NEXT) complex, linking to RNA decay. To dissect ZC3H18 function, we conducted interaction screening and mutagenesis of the protein, which revealed a phosphorylation-dependent isoform. Surprisingly, the modified region of ZC3H18 associates with core histone proteins. Further examination of ZC3H18 function, by genome-wide analyses, demonstrated its impact on transcription of a subset of protein-coding genes. This activity requires the CBC-interacting domain of the protein, with some genes being also dependent on the NEXT- and/or histone-interacting domains. Our data shed light on the domain requirements of a protein positioned centrally in nuclear RNA metabolism, and they suggest that post-translational modification may modulate its function.

SUBMITTER: Winczura K

PROVIDER: S-EPMC5770337 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Characterizing ZC3H18, a Multi-domain Protein at the Interface of RNA Production and Destruction Decisions.

Winczura Kinga K Schmid Manfred M Iasillo Claudia C Molloy Kelly R KR Harder Lea Mørch LM Andersen Jens S JS LaCava John J Jensen Torben Heick TH

Cell reports 20180101 1

Nuclear RNA metabolism is influenced by protein complexes connecting to both RNA-productive and -destructive pathways. The ZC3H18 protein binds the cap-binding complex (CBC), universally present on capped RNAs, while also associating with the nuclear exosome targeting (NEXT) complex, linking to RNA decay. To dissect ZC3H18 function, we conducted interaction screening and mutagenesis of the protein, which revealed a phosphorylation-dependent isoform. Surprisingly, the modified region of ZC3H18 as ...[more]

PMID: 29298432

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Dataset Information

Characterizing ZC3H18, a Multi-domain Protein at the Interface of RNA Production and Destruction Decisions.

Publications

Characterizing ZC3H18, a Multi-domain Protein at the Interface of RNA Production and Destruction Decisions.

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