ITRAQ-Based Proteomic Analysis of Sublethally Injured Escherichia coli O157:H7 Cells Induced by High Pressure Carbon Dioxide.

ABSTRACT: High pressure carbon dioxide (HPCD) could cause sublethally injured cells (SICs), which may cause food poisoning and spoilage during food storage and limit its application. Therefore, the formation of SICs of Escherichia coli O157:H7 was investigated by isobaric tag for relative and absolute quantification (iTRAQ) proteomic methods in this study for better controlling the SICs induced by HPCD. A total of 2,446 proteins was identified by iTRAQ, of which 93 and 29 were significantly differentially expressed in the SICs compared with live control cells (CK_L) and dead control cells (CK_D), respectively. Among the 93 differentially expressed proteins (DEP) in the SICs compared with CK_L, 65 proteins showed down-regulation and 28 showed up-regulation. Accord

SUBMITTER: Bi X 

PROVIDER: S-EPMC5770692 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature