Single-Cell Defects Cause a Long-Range Mechanical Response in a Confluent Epithelial Cell Layer.

ABSTRACT: Epithelial cells are responsible for tissue homeostasis and form a barrier to maintain chemical gradients and mechanical integrity. Therefore, rapid wound closure is crucial for proper tissue function and restoring homeostasis. In this study, the mechanical properties of cells surrounding a single-cell wound are investigated during closure of the defect. The single-cell wound is induced in an intact layer using micropipette action and responses in neighboring cells are monitored with atomic force microscopy. Direct neighbors reveal a rise in the apparent pretension, which is dominated by cortical tension. The same effect was observed for a single-cell wound induced by laser ablation and during closure of a not fully confluent layer. Moreover, changes in the apparent pretension are far reac

SUBMITTER: Karsch S 

PROVIDER: S-EPMC5770989 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature