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Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells.

ABSTRACT: In cryogenic correlated light and electron microscopy (cryo-CLEM), frozen targets of interest are identified and located on EM grids by fluorescence microscopy and then imaged at higher resolution by cryo-EM. Whilst working with these methods, we discovered that a variety of mammalian cells exhibit strong punctate autofluorescence when imaged under cryogenic conditions (80?K). Autofluorescence originated from multilamellar bodies (MLBs) and secretory granules. Here we describe a method to distinguish fluorescent protein tags from these autofluorescent sources based on the narrower emission spectrum of the former. The method is first tested on mitochondria and then applied to examine the ultrastructural variability of secretory granules within insulin-secreting pancreatic beta-cell-derived INS-1E cells.

SUBMITTER: Carter SD

PROVIDER: S-EPMC5771259 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells.

Carter Stephen D SD Mageswaran Shrawan K SK Farino Zachary J ZJ Mamede João I JI Oikonomou Catherine M CM Hope Thomas J TJ Freyberg Zachary Z Jensen Grant J GJ

Journal of structural biology 20171025 1

In cryogenic correlated light and electron microscopy (cryo-CLEM), frozen targets of interest are identified and located on EM grids by fluorescence microscopy and then imaged at higher resolution by cryo-EM. Whilst working with these methods, we discovered that a variety of mammalian cells exhibit strong punctate autofluorescence when imaged under cryogenic conditions (80 K). Autofluorescence originated from multilamellar bodies (MLBs) and secretory granules. Here we describe a method to distin ...[more]

PMID: 29078993

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