Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC5771714 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Chen Jianwei J Jiang Xiqian X Zhang Chengwei C MacKenzie Kevin R KR Stossi Fabio F Palzkill Timothy T Wang Meng C MC Wang Jin J
ACS sensors 20170818 9
We report a mitochondria-specific glutathione (GSH) probe-designated as Mito-RealThiol (MitoRT)-that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the ...[more]