Unknown

Dataset Information

0

Reversible Reaction-Based Fluorescent Probe for Real-Time Imaging of Glutathione Dynamics in Mitochondria.


ABSTRACT: We report a mitochondria-specific glutathione (GSH) probe-designated as Mito-RealThiol (MitoRT)-that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the cytosol not only under normal growing conditions but also upon oxidative stress.

SUBMITTER: Chen J 

PROVIDER: S-EPMC5771714 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Reversible Reaction-Based Fluorescent Probe for Real-Time Imaging of Glutathione Dynamics in Mitochondria.

Chen Jianwei J   Jiang Xiqian X   Zhang Chengwei C   MacKenzie Kevin R KR   Stossi Fabio F   Palzkill Timothy T   Wang Meng C MC   Wang Jin J  

ACS sensors 20170818 9


We report a mitochondria-specific glutathione (GSH) probe-designated as Mito-RealThiol (MitoRT)-that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the  ...[more]

Similar Datasets

| S-EPMC4371605 | biostudies-literature
| S-EPMC9066615 | biostudies-literature
| S-EPMC4037373 | biostudies-literature
| S-EPMC8163343 | biostudies-literature
| S-EPMC5998144 | biostudies-literature
| S-EPMC5511354 | biostudies-literature
| S-EPMC5466677 | biostudies-literature
| S-EPMC9214870 | biostudies-literature
| S-EPMC8905922 | biostudies-literature
| S-EPMC6486671 | biostudies-literature