Sensitive detection of fluorescence in western blotting by merging images.
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ABSTRACT:
SUBMITTER: Kondo Y
PROVIDER: S-EPMC5774814 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
Sensitive detection of fluorescence in western blotting by merging images.
Kondo Yukari Y Higa Shinichiro S Iwasaki Takeshi T Matsumoto Tomoya T Maehara Kazumitsu K Harada Akihito A Baba Yoshihiro Y Fujita Masatoshi M Ohkawa Yasuyuki Y
PloS one 20180119 1
The western blotting technique is widely used to analyze protein expression levels and protein molecular weight. The chemiluminescence method is mainly used for detection due to its high sensitivity and ease of manipulation, but it is unsuitable for detailed analyses because it cannot be used to detect multiple proteins simultaneously. Recently, more attention has been paid to the fluorescence detection method because it is more quantitative and is suitable for the detection of multiple proteins ...[more]
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