Cellular processing of gold nanoparticles: CE-ICP-MS evidence for the speciation changes in human cytosol.

ABSTRACT: The cellular uptake of gold nanoparticles (AuNPs) may (or may not) affect their speciation, but information on the chemical forms in which the particles exist in the cell remains obscure. An analytical method based on the use of capillary electrophoresis hyphenated with inductively coupled plasma mass spectrometry (ICP-MS) has been proposed to shed light on the intracellular processing of AuNPs. It was observed that when being introduced into normal cytosol, the conjugates of 10-50 nm AuNPs with albumin evolved in human serum stayed intact. On the contrary, under simulated cancer cytosol conditions, the nanoconjugates underwent decomposition, the rate of which and the resulting metal speciation patterns were strongly influenced by particle size. The new peaks that appeared in ICP-MS electr

SUBMITTER: Legat J 

PROVIDER: S-EPMC5775379 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature