Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells.
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ABSTRACT:
SUBMITTER: Deleye L
PROVIDER: S-EPMC5775416 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells.
Deleye Lieselot L Vander Plaetsen Ann-Sophie AS Weymaere Jana J Deforce Dieter D Van Nieuwerburgh Filip F
Scientific reports 20180119 1
To allow multiple genetic analyses on a single cell, whole genome amplification (WGA) is required. Unfortunately, studies comparing different WGA methods for downstream human identification Short Tandem Repeat (STR) analysis remain absent. Therefore, the aim of this work was to assess the performance of four commercially available WGA kits for downstream human identification STR profiling on a B-lymphoblastoid cell line. The performance was assessed using an input of one or three micromanipulate ...[more]
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