Unknown

Dataset Information

0

Alpha-actinin of the chlorarchiniophyte Bigelowiella natans.


ABSTRACT: The genome of the chlorarchiniophyte Bigelowiella natans codes for a protein annotated as an ?-actinin-like protein. Analysis of the primary sequence indicate that this protein has the same domain structure as other ?-actinins, a N-terminal actin-binding domain and a C-terminal calmodulin-like domain. These two domains are connected by a short rod domain, albeit long enough to form a single spectrin repeat. To analyse the functional properties of this protein, the full-length protein as well as the separate domains were cloned and isolated. Characerisation showed that the protein is capable of cross-linking actin filaments into dense bundles, probably due to dimer formation. Similar to human ?-actinin, calcium-binding occurs to the most N-terminal EF-hand motif in the calmodulin-like C-terminal domain. The results indicate that this Bigelowiella protein is a proper ?-actinin, with all common characteristics of a typical ?-actinin.

SUBMITTER: Backman L 

PROVIDER: S-EPMC5775757 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

altmetric image

Publications

Alpha-actinin of the chlorarchiniophyte <i>Bigelowiella natans</i>.

Backman Lars L  

PeerJ 20180117


The genome of the chlorarchiniophyte <i>Bigelowiella natans</i> codes for a protein annotated as an α-actinin-like protein. Analysis of the primary sequence indicate that this protein has the same domain structure as other α-actinins, a N-terminal actin-binding domain and a C-terminal calmodulin-like domain. These two domains are connected by a short rod domain, albeit long enough to form a single spectrin repeat. To analyse the functional properties of this protein, the full-length protein as w  ...[more]

Similar Datasets

| S-EPMC7883536 | biostudies-literature
| PRJNA27851 | ENA
| PRJNA17831 | ENA
| PRJNA16077 | ENA
| PRJNA27935 | ENA
| PRJDB4720 | ENA
| S-EPMC5635652 | biostudies-literature
2019-01-01 | GSE115762 | GEO
| PRJNA47111 | ENA
| PRJNA488211 | ENA