Dataset Information

Interleukin-18 serum level is elevated in type 2 diabetes and latent autoimmune diabetes.

ABSTRACT:

Background

Interleukin-18 (IL-18) is an inflammatory cytokine found to be elevated in obesity, metabolic syndrome and type 2 diabetes (T2D) as a part of the chronic low-grade inflammatory process in these states. The aim of the study was to evaluate the interleukin level in patients with latent autoimmune diabetes of the adults (LADA) in comparison to that in T2D subjects.

Materials and methods

IL-18 was analyzed through enzyme-linked immunosorbent assay in 76 participants with T2D and 24 with LADA and 14 control subjects. Evaluation was also carried out in body mass index (BMI)- and glycemic control-matched diabetic patients.

Results

The serum concentration of IL-18 was higher in patients with T2D (389.04?±?203.44?pg/mL) and LADA (327.04?±?144.48?pg/mL) than that in control subjects (219.88?±?91.03?pg/mL), P?P?=?0.255) despite higher IL-6 (4.78?±?5.84 vs 1.79?±?0.96?pg/mL, P?P?=?0.002) level in T2D patients. The results were persistent in BMI-matched subjects with diabetes (IL-18?=?403.48?±?226.32 vs 329.30?±?146.30?pg/mL, respectively for T2D and LADA, P?=?0.391). The correlations in T2D group concerning HDL cholesterol (r?=?-0.377, P?=?0.001), postprandial glucose (r?=?0.244, P?=?0.043), IL-6 (r?=?0.398, P?r?=?0.427, P?=?0.001) were not confirmed in LADA and control subjects.

Conclusion

The IL-18 serum level was higher in T2D and LADA than that in control subjects, but did not differ between both diabetic groups, even when they were BMI matched. Correlations with lipid, glycemic and inflammatory parameters were present in T2D only.

SUBMITTER: Zaharieva E

PROVIDER: S-EPMC5776671 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Interleukin-18 serum level is elevated in type 2 diabetes and latent autoimmune diabetes.

Zaharieva Emanuela E Kamenov Zdravko Z Velikova Tsvetelina T Tsakova Adelina A El-Darawish Yosif Y Okamura Haruki H

Endocrine connections 20171207 1

<h4>Background</h4>Interleukin-18 (IL-18) is an inflammatory cytokine found to be elevated in obesity, metabolic syndrome and type 2 diabetes (T2D) as a part of the chronic low-grade inflammatory process in these states. The aim of the study was to evaluate the interleukin level in patients with latent autoimmune diabetes of the adults (LADA) in comparison to that in T2D subjects.<h4>Materials and methods</h4>IL-18 was analyzed through enzyme-linked immunosorbent assay in 76 participants with T2 ...[more]

PMID: 29217651

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Project description:BackgroundHigh circulating interleukin (IL)-18 level predicts a higher hospitalization rate among dialysis patients, possibly through cardiovascular mechanisms; however, whether higher IL-18 level is associated with mortality in dialysis patients is less clear. In addition, its impacts on left ventricular (LV) function are also unknown. We conducted a cohort study to examine the impacts of IL-18 level on LV function and prognosis among clinically stable hemodialysis patients.MethodsClinically stable patients undergoing maintenance hemodialysis (? 3 months) were prospectively enrolled from December 2008 to January 2009, and were followed up for 31 months. The enrolled patients (41% male, 66.4 ± 10.9 years of age) received 2-dimensional echocardiography and myocardial deformation (strain) analysis, including LV peak systolic longitudinal strain (GLS) and circumferential strain (CS). Laboratory measurements were also performed. Cox regression analysis was used to investigate prognostic factors.ResultsSeventy-five patients were stratified into 2 groups by the median value of IL-18 (654.2 pg/ml). Between these 2 groups, there was no significant difference in baseline characteristics including LV ejection fraction. The high IL-18 group had a worse LV systolic function as demonstrated by reduced GLS and CS. Seventeen patients (22.7%) died during the follow-up period. Multivariate Cox regression analysis showed that low serum albumin, the presence of hypertension, high serum IL-18, and less negative GLS (>-15%) were independently associated with all-cause mortality. No significant interaction between IL-18 and less negative GLS was noted in the final Cox model.ConclusionHemodialysis patients with high IL-18 levels tend to have worse LV systolic function and higher mortality rate. However, elevated serum IL-18 level is predictive of poor prognosis among stable hemodialysis patients, independently of LV dysfunction. This suggests an additional value of IL-18 to echocardiographic study in predicting all-cause mortality, and IL-18 may be helpful in early risk stratification of hemodialysis patients.

Project description:Background:Recent studies have suggested that cell death might be involved in the pathophysiology of metabolic disorders. The cytokeratin 18 (CK18) fragment, as a cell death marker, plays an important role in nonalcoholic fatty liver disease (NAFLD). However, only a limited number of studies have found elevated serum levels of CK18 in patients with type 2 diabetes. Moreover, no studies have been conducted yet to investigate the role of CK18 in hypertension or dyslipidemia. In particular, CK18 M65ED is a more sensitive marker of cell death, and its role in cardiometabolic disorders has not been revealed yet. Methods:A total of 588 subjects were enrolled from the local communities of Shanghai. Serum CK18 M65ED were determined using the enzyme-linked immunosorbent assay. A cardiometabolic disorder was identified by the presence of at least one of the components including overweight or central obesity, diabetes, dyslipidemia, and hypertension. Results:Subjects with cardiometabolic disorders exhibited significantly higher serum levels of CK18 M65ED than those without cardiometabolic disorders (197.36 (121.13-354.50) U/L versus 83.85 (52.80-153.75) U/L, respectively, P < 0.001). Increased serum CK18 M65ED quartiles were associated with the increased prevalence of cardiometabolic disorders and its components (P < 0.001 for all components). Multiple stepwise regression analysis also revealed that diastolic blood pressure, glycated hemoglobin A1c, alanine transaminase, and high-density lipoprotein cholesterol were independently correlated with serum CK18 M65ED levels (all P < 0.01). In addition, logistic regression analysis showed that the serum CK18 M65ED levels were positively correlated with cardiometabolic disorders and in an independent manner. Further, CK18 M65ED was revealed to be an indicator of cardiometabolic disorders in a NAFLD-independent manner. Conclusions:Elevated levels of CK18 M65ED, a sensitive cell death marker, were independently and positively correlated with cardiometabolic disorders, even after the adjustment for the presence of NAFLD and other cardiovascular risk factors.

Dataset Information

Interleukin-18 serum level is elevated in type 2 diabetes and latent autoimmune diabetes.

Background

Materials and methods

Results

Conclusion

Publications

Interleukin-18 serum level is elevated in type 2 diabetes and latent autoimmune diabetes.

Similar Datasets

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